BBa_K1615020

BBa_K1615020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1615020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brooke Rothschild-Mancinelli
Date created: 2015-08-26 11:00:00
Date modified: 2015-08-27 05:19:10

Linker-CBDcipA-linker fused to MorA driven by LacI promoter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1615020_sequence (Version 1)

Description

Morphine-6-dehydrogenase fused C terminally to CBDcipA with N and C terminal linkers driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

Morphine-6-dehydrogenase from Pseudomonas putida. CBDcipA from Clostridium thermocellum. Lac expression cassette from iGEM10_Washington.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1615020/1