BBa_K1615064

BBa_K1615064 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1615064
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brooke Rothschild-Mancinelli
Date created: 2015-08-27 11:00:00
Date modified: 2015-08-28 09:05:46

HerE fused to CBDclos driven by LacI promoter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1615064_sequence (Version 1)

Description

Heroin Esterase fused N terminally to CBDclos driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

Heroin Esterase from Rhodococcus erythropolis. CBDclos from Clostridium cellulovorans. Lac expression cassette from iGEM10_Washington.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1615064/1