BBa_K1624000

BBa_K1624000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1624000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: LASATX iGEM team
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-12 02:15:53

CO detector GFP reporter system



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K1624000_sequence (Version 1)

Description

pCoof promoter followed by ribosome binding site. Can be placed upstream of genes to regulate gene expression in response to the presence of carbon monoxide. Works together with the CooA transcription activator, which activates pCoof promoter once bound to carbon monoxide.

Notes

Created primer with part as an extension.

Source

pCoof promoter from Rhodospirillum rubrum. Sequence from http://www.jbc.org/content/274/16/10840.full.pdf and BBa_K352002.

Sequence Annotation Location Component / Role(s)
pCoof
RBS
sfGFP
BBa_K352002
BBa_K1624003
BBa_K1624004
1,94
104,110
118,861
1,94
104,110
118,861
promoter feature/promoter
ribosome_entry_site feature/rbs
feature/protein CDS
engineered_region feature/BioBrick
engineered_region feature/BioBrick
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
No part sequence
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1624000/1