Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandro Gutierrez
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-11 02:15:10
Device to demethylate 1-methylxanthine
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1627000_sequence (Version 1) |
Description
This part contains genes that should allow for the demethylation of 1-methylxanthine. The part contains a synthetic operon consisting of ndmA, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.While this device should be able to demethylate the 1-methyl group on methylxanthines, the ndmA gene lacks specificity to 1-methylxanthine. Thus, this part is not actually able to be used as designed. However, this part was important for the construction of parts BBa_K1627003, BBa_K1627004, and BBa_K1627006, which are able to demethylate the 1-methylgroup from theophylline, paraxanthine, and caffeine, respectively.
Notes
These genes were amplified from the pDCAF3 plasmid (BBa_K734000). A PstI restriction site was removed from the ndmA gene during the cloning process. The genes were put together into the pSB1C3 backbone using BioBrick Standard Assembly.Source
The ndmA and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.| Sequence Annotation | Location | Component / Role(s) |
| J23110 K515105 ndmA B0032 ndmD B0034 gst9 | 1,35 44,68 69,1124 1133,1145 1152,2918 2963,2974 2981,3655 | feature/promoter promoter ribosome_entry_site feature/rbs feature/cds CDS ribosome_entry_site feature/rbs CDS feature/cds feature/rbs ribosome_entry_site CDS feature/cds |