BBa_K1633004

BBa_K1633004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1633004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 08:03:05

MOR siRNA-2 (siRNA for mouse Mu opioid receptor)



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K1633004_sequence (Version 1)

Description

This part is a short hairpin RNA (shRNA) sequence. When this shRNA sequence is cut by restriction enzyme and then integrated into pcDNA 6.2 vector, this shRNA can play a RNAi function in mammalian cell lines such as HEK293 cell. When the shRNA vector of MOR is transfected into HEK293 cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at Mus musculus MOR mRNA under the guide of siRNA sequence and cleave the MOR mRNA.

Notes

We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid.

Source

We designed the MOR siRNA-2 and ordered the part from a DNA synthesis company.

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BBa_K1633004/1