| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1634003_sequence (Version 1)
|
Description
ChR2 is a kind of channelrhodopsin. Channelrhodopsins are a subfamily of retinylidene proteins (rhodopsins) that function as light-gated ion channels.[1] They serve as sensory photoreceptors in unicellular green algae, controlling phototaxis: movement in response to light.[2] When we express the channelrhodopsins in some specific cells in organisms and shed specific light on them, we can activate or supress the specific ion channel to change the activity of the cell. And if the cell is a muscle cell or a neuron, this tiny change may trigger a great change in the organism's activity. To make it easier to confirm the location and expression level of the ChR2, we construct a fusion protein of ChR2 and YFP.
Notes
Since we use the restriction enzyme site to do the ligation, we should pay attention to the sequence of ChR2-YFP, avoiding using the restriction site exits in it.
Source
ChR2 is a channelrhodopsin in green algae, but we didn't get it directly from the algae. Instead, we get ChR2 from some plamids by PCR.
