BBa_K1641000

BBa_K1641000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1641000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pai Li
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 01:59:04

Recombinase Vcre, E. coli-and-yeast coding optimized



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1641000_sequence (Version 1)

Description

This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system. Vcre has the invertase activity that invert the the sequence between two anti-parallel Vloxp.
The original Vcre is discovered through genome screening of Vibrio sp. 0908, yet this coding sequence is redesigned into E. coli-and-yeast coding preference from original protein sequence.

Notes

Optimized for both E. coli and S. cerevisiae, but neither is most perfectly fitted.

Source

This recombinase comes from de novo gene synthesis.

Sequence Annotation Location Component / Role(s)
Vcre coding
1,1146
feature/cds CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1641000/1