BBa_K1641003

BBa_K1641003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1641003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pai Li
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 03:42:02

Recombinase Vika, E. coli and yeast coding optimized



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1641003_sequence (Version 1)

Description

This is a Cre-like recombinase of Tyr-family with properties similar to those of Cre, but with a different recognized sequence VloxP, which do not cross-interfering with Cre-LoxP system.

Vika has the invertase activity that invert the the sequence between two anti-parallel Voxs. The original Vika is discovered through genome screening of Vibrio coralliilyticus ATCC BAA-450, which might be a integrase gene taken in by some phage. Yet this coding sequence is redesigned into E. coli-and-yeast coding preference according to original protein sequence.

Notes

This recombinase is both E. coli and S. cerevisiae coding optimized, but neither is perfectly fitted.

Source

De novo synthesis.

Sequence Annotation Location Component / Role(s)
Vika coding
1,1089
feature/cds CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1641003/1