BBa_K1641006

BBa_K1641006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1641006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pai Li
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-13 10:53:02

Fusion protein of Vcre::EGFP::ssra-tag, with RBS at beginning



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K1641006_sequence (Version 1)

Description

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Vcre::EGFP::ssra-tag. The part is for expression of Vcre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.

Vcre (BBa_1641000) in our bricks is a new-discovered Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel VloxP sites up-side-down. A EGFP fusion at the C-term of Vcre will not significantly interfere the enzymatic activity. A ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression.

Notes

Ssra-Tag is efficiently cleaning the protein when not under induction, and helps to reduce leakage expression. Vcre is according to its structure is similar to Cre, and hence the C-term EGFP fusion tend not to interfere the activity.

Source

Vcre from BBa_1641000 (de novo synthesized gene) and EGFP::ssra-tag from pBI121-EGFP is fused by overlap-PCR; others through connection of bricks.

Sequence Annotation Location Component / Role(s)
B0034
Vcre
Flexible chain
EGFP::ssra
1,12
19,1158
1159,1182
1183,1941
feature/rbs ribosome_entry_site
CDS feature/protein
feature/misc sequence_feature
feature/protein CDS
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1641006/1