BBa_K1641015

BBa_K1641015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1641015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 08:03:56

Up-side-down RBS::mcherry-ssra with FRT recognition sites

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1641015_sequence (Version 1)

Description

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two FRT sites at the upstream and downstream of the gene. FRT can be recognized and the middle sequence can be restored by invertase Flp. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without start code and restriction enzyme activity. This sequence is absolutely compatible with brick construction method and usually less likely to make trouble.

Notes

This sequence is up-side-down, hence can be inverted into normal sequence.

Source

This part is constructed by PCR from biobricks or cut-and-link.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
FRT	1,34	feature/binding, non_covalent_binding_site
inverted mcherry-ssra	44,787	CDS, feature/cds
B0034	794,805	ribosome_entry_site, feature/rbs
FRT	812,845	feature/binding, non_covalent_binding_site

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1641015/1

Member of these Collections

• iGEM Parts Registry