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BBa_K1641018

BBa_K1641018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1641018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pai Li

Date created: 2015-09-09 11:00:00

Date modified: 2015-09-10 11:58:35

Up-side-down RBS::mcherry-ssra with SloxM1, Scre recognition sites

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Details

Types	DnaRegion
Roles	engineered_region Translational_Unit
Sequences	BBa_K1641018_sequence (Version 1)

Description

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two SloxM1 sites at the upstream and downstream of the gene. SloxM1 can be recognized by invertase Scre and the middle mcherry can be restored through inversion. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Notes

This sequence is up-side-down, hence can be inverted into normal sequence.

Source

This part is constructed by PCR or cut-and-link of biobricks.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SloxM1 inverted mcherry-ssra B0034 SloxM1	1,34 44,787 794,805 812,845	non_covalent_binding_site feature/binding feature/cds CDS ribosome_entry_site feature/rbs feature/binding non_covalent_binding_site