Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pai Li
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-12 03:51:10
Reporter of Invertase activity of Cre, pInv-rep-100LoxM
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1641024_sequence (Version 1) |
Description
This is the reporter of inversion activity of invertase Cre. An up-side-down mcherry gene with RBS is surrounded by a pair of LoxP and controlled by a constructive promoter BBa_J23101. At first stage, due to the meaningless mRNA, red signal cannot be generated, but when Cre exist,it inverts and restore the mcherry gene between two LoxP, the red signal can be generated at once.This device is constructed to measure the real-time dynamics of timing module of Cre in the Micro-timer presented by SYSU-CHINA.
Notes
We aim to construct a real-timer detector of invertase dynamics, which must be quantifiable. Hence, using mcherry, one of the best fluorescence signal, we can supervise the RFU of a strain and precisely simulate the exact situation of each invertase timing module. The data can be valuable for modeling work and the inversion time of corresponding module can be calculated.Source
All of the parts come from biobricks.| Access | Instance | Definition |
| public | BBa_J23101 | BBa_J23101 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 | 1,35 | BBa_J23101 |