Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pai Li
Date created: 2015-09-11 11:00:00
Date modified: 2015-09-12 04:00:41
Reporter of Invertase activity of Cre, pInv-rep-110LoxM
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1641026_sequence (Version 1) |
Description
This is a reporter of invertase activity of Cre for Real-time measurement of dynamics of timing modules by SYSU-CHINA 2015.The target sequence LoxP of Cre locates in the pInv-rep, surrounding a mcherry gene which is yet upside-down and transcribed by a constructive promoter. This mcherry-coding sequence can be inverted and restored to 5??? ??? 3??? direction at the existence of Cre or its EGFP fusion, rendering red signal. By real-time measurement of EGFP and mcherry, we can obtain data of Cre dynamics and simulate this process through modelling.
For this reporter:
RTS type: LoxP;
mcherry type: BBa_J06504-BBa_M0052, which is mcherry-ssra(moderately fast);
Promoter type: BBa_J23106;
Notes
All reporter is constructed as following pattern: Constructive promoter :: RTS :: up-side-down RBS-mcherry-(ssra) :: RTS :: terminatorSource
This brick is constructed through cut-and-link of other bricks, without risky sources.| Access | Instance | Definition |
| public public public | BBa_J23110 BBa_K1641014 BBa_B0015 | BBa_J23110 BBa_K1641014 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_K1641014 BBa_B0015 | 1,35 44,888 897,1025 | BBa_J23110 BBa_K1641014 BBa_B0015 |