Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peivand Sadat Mousavi
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 07:08:30
Dual Color Plasmid (RFP and GFP)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1645999_sequence (Version 1) |
Description
This part is the composite part of GFP full cassette taken from registry part BBa_I20260 and RFP full cassette taken from registry part BBa_J04450. The GFP is under the J23101 promoter, which is a constitutive promoter. In addition, the RFP is under the lacI (R0010) promoter which is constitutive in DH5-alpha due to the absence of lacI expression. For our purposes, this part was used to see the colour swaps using different SgRNA targeting promoter region of each of the colours.Notes
First BBa_J04450 was cloned into BBa_I20260 using XbaI and PstI. Then the backbone was changed from pSB3K3 into pSB1C3.Source
This part is constructed using two existing registry parts, BBa_I20260, BBa_J04450.| Access | Instance | Definition |
| public public | BBa_I20260 BBa_J04450 | BBa_I20260 BBa_J04450 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I20260 BBa_J04450 | 1,919 928,1996 | BBa_I20260 BBa_J04450 |