Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leung King Pong
Date created: 2015-07-06 11:00:00
Date modified: 2015-07-25 12:43:34
Recombination Template for mamAB Operon
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1648000_sequence (Version 1) |
Description
This part is constructed by four fragments from the magnetospirillum gryphiswaldense magnetosome island, and it is to be used to do the homologous recombination with the genomic DNA of the magnetospirillum to form the whole coding sequence ofNotes
In order to form a homologous recombination template, we select 250 bp of each fragment and do the overlapping PCR ligate fragments together.Source
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome| Sequence Annotation | Location | Component / Role(s) |
| mamH 5' flanking sequence mamN 3' flanking sequence mamO 5' flanking sequence mamU 3' flanking sequence | 1,250 251,500 521,770 771,1020 | feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature |