Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YANG YUANZHAN
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-16 02:48:32
PAI1 detector
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1651000_sequence (Version 1) |
Description
When the PAI1 spread into the amplifier, it will combine with a protein named lasR which is regulated by a constitutive promoter to form a complex. The PAI1-lasR complex will then activate the pLAS to produce a protein named rhlI.The rhlI will catalyze the production of PAI2, which will combine with another protein coming from the rhlIR circuit. And the rhlR is also regulated by a constitutive promoter like lasR. Now there comes to the PAI2-rhlR complex.The PAI2-rhlR complex will directly activate the pRHL to enable the production of super - folder GFP.
Notes
There are two systems including LasIR and RhlIR existing in the bacterium. So what is considered about is to make a streamline design of the two-stage amplification element. Meanwhile, the core regulatory element is also restained.Source
Series group effect element P.Aeruginosa.| Access | Instance | Definition |
| public public public public | BBa_K553003 BBa_R0079 BBa_I9026 BBa_K575032 | BBa_K553003 LasR+PAI BBa_I9026 BBa_K575032 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K553003 BBa_R0079 BBa_I9026 BBa_K575032 | 1,921 930,1086 1095,1779 1788,3390 | BBa_K553003 LasR+PAI BBa_I9026 BBa_K575032 |