BBa_K1669003

BBa_K1669003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1669003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nina Jerala

Date created: 2015-09-12 11:00:00

Date modified: 2015-09-13 02:03:36

CtfB + His-tag

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1669003_sequence (Version 1)

Description

This part includes the CtfB gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli.
This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA.
The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes.

Notes

C-terminal His-tag.

Source

Sequence retrieved from GenBank and codon optimized for expression in E. coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG	1,3	feature/start, start_codon
CtfB	1,673	CDS, feature/cds
His-tag	674,682	feature/tag, tag
double stop codon	683,687	stop_codon, feature/stop

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1669003/1

Member of these Collections

• iGEM Parts Registry