BBa_K1676084

BBa_K1676084 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1676084
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-25 02:15:26

Mutant 8 of LDH and wildtype LDP



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1676084_sequence (Version 1)

Description

This construct is an operon for expression of our mutant LDH and wildtype LDP.

Notes

Primers were designed to have the start and stop codon so that they will not be mutated during PCR

Source

Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1676084/1