BBa_K1677100

BBa_K1677100 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1677100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mackenzie Labine-Romain

Date created: 2015-08-05 11:00:00

Date modified: 2015-08-20 06:36:11

Listeriolysin (lactococcus)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1677100_sequence (Version 1)

Description

Listeriolysin O (LLO) is a hemolysin that forms pores in phagosomes, and allows the bacterium expressing it to escape into the cytoplasm without damaging the plasma membrane.

This part was adapted from BBa_K299812 (Listeriolysin) and codon optimised for Lactococcus species.

Notes

This part was assembled by overlap PCR of two IDT gBlocks, and certain codons were changed to allow for this format of DNA synthesis.

Source

The LLO gene was originally source by the Warsaw 2010 team from the bacterium Listeria monocytogenes.

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1677100/1

Member of these Collections

• iGEM Parts Registry