BBa_K1677391

BBa_K1677391 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1677391

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Isabelle Capell-Hattam

Date created: 2015-08-24 11:00:00

Date modified: 2015-08-25 01:40:08

IBV Pseudoknot

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_K1677391_sequence (Version 1)

Description

This is part of the BABS iGEM pseudoknot suite. This pseudoknot can be used for delayed translation of a protein sequence, by slowing the ribosome.

Notes

All start codons had to be removed from the pseudoknot.

20 base pairs from the viral DNA flank either side of the pseudoknot region.

3' flanking sequence AGAATTATTTAAACGGGTAC

5' flanking sequence TTTGAGGTTTGTAATAAGGA


The actual pseudoknot is:

GGGGTAGCAGTGAGGCTCGGCTGATACCCCTTGCTAGTGGGTGTGACCCTGATATTGTAAAGCGAGCC

((((((.((((..[[[[[[)))).))))))((((....((((...)))).......))))..]]]]]]

Estimated free energy: -32.02 kcal/mol

Source

From the infectious bronchitis virus.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
3' Flanking Region	1,20	feature/misc, sequence_feature
5' Flanking Region	88,108	sequence_feature, feature/misc
IBV Pseudoknot structure	21,87	feature/tag, tag

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1677391/1

Member of these Collections

• iGEM Parts Registry