BBa_K1678000

BBa_K1678000 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1678000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Antoine Vigouroux

Date created: 2015-09-07 11:00:00

Date modified: 2015-09-16 09:44:49

Promoter from BBa_J23119 followed by 4 orthogonal LoxP sites

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1678000_sequence (Version 1)

Description

This is a strong constitutive promoter followed with four orthogonal LoxP sites, that can be used for constructing brainbow-like systems in E. coli.

Notes

Fillers are present in between the LoxP sites to make it easier to synthesize and allow for PCR primers design.

Source

Gene synthesis.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LoxP	2094,2127	feature/misc, sequence_feature
BBa_J23119 promoter	2051,2085	feature/promoter, promoter
Lox2272	2136,2169	sequence_feature, feature/misc
LoxN	2182,2215	sequence_feature, feature/misc
Lox5171	2225,2258	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1678000/1

Member of these Collections

• iGEM Parts Registry