Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Barthelemy Caron
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 06:54:59
RibT promoted by p25 synthetic promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1678002_sequence (Version 1) |
Description
Bacillus subtilis RibT gene for riboflavin production. RibT codes for the Beta-chain of the Riboflavin synthase. Coding sequence has been codon optimized for Lactobacillus plantarum.Protein is expressed by L.plantarum synthetic promoter p25 (Design description in 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology (2006), 152, 1011???1019.).
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
The expressed protein catalyses one step in the riboflavin pathway.
ArPP+ DHBP -> DRL
Notes
noneSource
CDS sequence is coming from the genomic sequence of Bacillus subtilis.| Sequence Annotation | Location | Component / Role(s) |
| p25 promoter RibT Tldh Termintator RBS | 22,81 97,471 472,609 82,88 | feature/promoter promoter feature/cds CDS feature/misc sequence_feature feature/rbs ribosome_entry_site |