Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cun Wei
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-18 09:15:45
promoter+RNA thermometer+RFP
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1679012_sequence (Version 1) |
Description
This is a device containing a promoter(BBa_J23106),a RBS(BBa_K115003) and a RFP coding sequence(BBa_E1010) on the pSB1C3 ,which can be easily transformed into E.coli.Then the E.coli would produce RFP when the temperature rises above 37??C.Notes
We use a special RBS(BBa_K115003) to make the device temperature-sensitive.When the temperature is below 37??C,the secondary structure of the RBS after transform would be folded.Thus the ribosome can't translated RFP.However,when the temperature rises above 42??C,the secondary structure of the RBS after transform would be unfolded and produce RFP.Source
BBa_J23106BBa_K115003
BBa_E1010
| Access | Instance | Definition |
| public public public | BBa_J23106 BBa_K115003 BBa_E1010 | BBa_J23106 BBa_K115003 mRFP1 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_K115003 BBa_E1010 | 1,35 44,152 159,864 | BBa_J23106 BBa_K115003 mRFP1 |