BBa_K1679022

BBa_K1679022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1679022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xihan Zhang
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 08:32:24

KIG, kcoil+intein+gfp



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1679022_sequence (Version 1)

Description

KIG is a fusion protein constructed from Kcoil, VMA and green fluorescent protein. Kcoil is a synthetic coiled-coil domain designed specifically to bind to Ecoil with high affinity and specificity. VMA is a thermosensitive intein. We design it to use cooperatively with BBa_K1189018(2013,Calgary).

Notes

KIG is a fusion protein without scar inside, constructed from Kcoil, VMA and green fluorescent protein. Kcoil is a synthetic coiled-coil domain designed specifically to bind to Ecoil with high affinity and specificity. VMA is a thermosensitive intein. We design it to use cooperatively with BBa_K1189018(2013,Calgary).

Source

Synthesized by Genescript.

Sequence Annotation Location Component / Role(s)
GFP
VMA
linker
kcoil
7,720
721,1314
1321,1368
1366,1428
CDS feature/protein
feature/protein CDS
feature/protein CDS
feature/tag tag
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1679022/1