dTomato

BBa_K1688004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1688004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Gullberg
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-18 10:34:03

dTomato (inc RBS)



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K1688004_sequence (Version 1)

Description

The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 and (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011).

Source

It was derived from a monomeric mutant of the anthozoan coral RFP, DsRed

Access Instance Definition
public
public
BBa_B0034
BBa_K1688019
BBa_B0034
dTomato
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1688004/1