BBa_K1689012

BBa_K1689012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1689012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiaofeng Li
Date created: 2015-09-15 11:00:00
Date modified: 2015-11-17 09:39:04

dCas9-Nlact



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K1689012_sequence (Version 1)

    Description

    NFluc of lactamase fused with dCas9, His tag allows for protein purification

    Notes

    goldengate

    Source

    E.coli

    Sequence Annotation Location Component / Role(s)
    His
    dCas9-Nlact
    dCas9
    GGGGS*2 Linker
    Nlact for N terminal of β-Lactamase
    start
    random spacer
    stop
    random spacer
    		37,54
    34,4701
    55,4155
    4156,4185
    4186,4698
    34,36
    1,33
    4699,4701
    4702,4772
    		tag feature/tag
    feature/cds CDS
    CDS feature/protein
    feature/protein CDS
    feature/protein CDS
    feature/start start_codon
    sequence_feature feature/misc
    feature/stop stop_codon
    feature/misc sequence_feature

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1689012/1
     

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