BBa_K1690005

BBa_K1690005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1690005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Sosnova, Pavel Zach

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 05:35:03

Pheromone receptor (C. parapsilosis)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1690005_sequence (Version 1)

Description

This is a coding sequence of C. parapsilosis mating pheromone receptor. Cells with this receptor being expressed react to the C. parapsilosis mating pheromone by the activation of the pheromone mating pathway.

The coding sequence was codon optimized for the expression in S. cerevisiae. C-terminal was replaced by a C-terminal from C. albicans for more efficient coupling to the mating pathway in S. cerevisiae.

Detailed description can be obtained from the iGEM 2015 Czech Republic wiki.

Notes

The coding sequence was codon optimized for the expression in S. cerevisiae. Kozak sequence was added before the start codon for a better translation. C-terminal was replaced by a C-terminal from C. albicans for more efficient coupling to the mating pathway in S. cerevisiae.

Source

The whole sequence was synthetized.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kozak	1,6	ribosome_entry_site, feature/rbs
start	7,9	feature/start, start_codon
Receptor	10,1206	CDS, feature/protein
stop	1207,1209	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1690005/1

Member of these Collections

• iGEM 2018 Distribution Plate 7
• iGEM 2018 Distribution Plate 7 Well 5D
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry