LuxI-His

BBa_K1709000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1709000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Astrid Deryckere
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-18 04:12:52

LuxI-His



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1709000_sequence (Version 1)

Description

Autoinducer synthetase for acyl-homoserine lactones (AHL):
LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate.
LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes.

The LuxI gene is fused to a His-tag facilitating gene expression measurements.

Notes

Biobrick BBa_C0061 still contained a Barcode sequence which was deleted.
For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.

Source

Sequence is originating from V. fischeri.
Here, the sequence from BBa_C0061 was used and optimized.

Sequence Annotation Location Component / Role(s)
ATG Start Codon
LuxI Coding Region
Polyhistidine-tag
Ochre Stop Codon
1,3
1,579
583,606
607,609
start_codon feature/start
feature/cds CDS
tag feature/tag
stop_codon feature/stop
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1709000/1