Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gregoire Thouvenin
Date created: 2015-08-09 11:00:00
Date modified: 2015-08-10 10:13:34
dCas9-ω
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1723000_sequence (Version 1) |
Description
This part codes for a enzymatically inactive Cas9 protein (dCas9) that is fused to rpoZ, the omega subunit of E.Coli RNA Polymerase (RNAP).Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease that targets and cleaves any DNA sequence complementary to its guide RNA (gRNA). Catalytically ???dead??? Cas9 (dCas9) lack the ability to cleave DNA. When fused to a RNAP recruiting element (in this case the omega subunit of RNAP), chimeric dCas9 can act as a programmable transcription activator. dCas9 may also act as a DNA transcription inhibitor: depending on its gRNA-determined binding site, it has been shown in yeasts to sterically hinder RNAP recruitment to promoter sequences.