R0011-N

BBa_K1725080 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1725080

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mhairi Davidson

Date created: 2015-08-10 11:00:00

Date modified: 2016-07-23 11:23:04

Promoter (lacI regulated, lambda pL hybrid) with extra Nhe I site

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1725080_sequence (Version 1)

Description

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be:
repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]).
induced by IPTG in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range

Extra Nhe1 site added, for ease of checking for BioBrick with a digest

Notes

BBa_R0011 with Nhe1 site upstream of promoter (after prefix)

Source

From BBa_R0011.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NheI site	1,6	sequence_feature, feature/misc
PLlac 0-1 promoter	7,61	feature/promoter, promoter

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1725080/1

Member of these Collections

• iGEM Parts Registry