BBa_K1725428

BBa_K1725428 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1725428

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrey Filipov

Date created: 2015-09-07 11:00:00

Date modified: 2015-09-16 07:37:03

J23114 + RBS + UirR + B0015

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1725428_sequence (Version 1)

Description

The UV intensity response regulator coding sequence was positioned downstream of J23114, a constitutive promoter family member, and upstream of the double terminator B0015. This allows the part to be placed in front of other parts with weaker or non-constitutive promoters.

Notes

The regulator and J23114 were ligated to B0015 after restriction that was followed by an Azure-A-based gel extraction.

Source

The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.

Components

Access	Instance	Definition
public	BBa_K1725424	BBa_K1725424
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1725424	1,788	BBa_K1725424
BBa_B0015	797,925	BBa_B0015

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1725428/1

Member of these Collections

• iGEM Parts Registry