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Trg-EnvZ

BBa_K1731000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1731000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alejandro Rodriguez-Gama, Lizbeth Airais Bola??os Castro

Date created: 2015-09-13 11:00:00

Date modified: 2015-09-16 02:00:58

Constitutive Trg-EnvZ Chimera

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K1731000_sequence (Version 1)

Description

The Chimera Trg-EnvZ is a two component system that constitutes a chimeric protein composed of the extracellular and transmembrane domains of chemoreceptor Trg and the cytosolic kinase domain of osmosensor EnvZ . Trg is activated by periplasmic binding proteins, and transduces the message to EnvZ to phosphorylate OmpR, a transcription factor which binds to the OmpC promoter driving transcription of whatever gene that is downstream.

Notes

Some restriction sites were removed without altering the open reading frame.

Source

Synthetic and codon optimized for E. coli expression

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23101 BBa_B0034 TrgZ BBa_B0015	1,35 45,56 63,1550 1560,1688	feature/promoter promoter ribosome_entry_site feature/rbs feature/cds CDS feature/BioBrick engineered_region