BBa_K1734004

BBa_K1734004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1734004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eduardo Ram??rez Montiel

Date created: 2015-09-17 11:00:00

Date modified: 2016-01-21 02:26:16

Nanoluc (Codon optimized for Sf9 cells)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1734004_sequence (Version 1)

Description

Nluc luciferase is an enzyme that was engineered to work as a luminescent reporter weighing 19.1 kDa. This protein uses fumarizine as novel substrate to produce a high intensity glow-type luminescence. These reactions are ATP-independent, trying to obtain the maximal assay sensitivity.




Reference:

[1] Promega (2015). Nanoluc Genetic Reporter Vectors. URL: http://worldwide.promega.com/products/reporter-assays-and-transfection/reporter-vectors-and-cell-lines/nanoluc-luciferase-reporters/nanoluc-genetic-reporter-vectors/

Notes

For our project purposes its use is destined to work as a reporter of protein expression

Source

Promega's product

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Nanoluc	1,525	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1734004/1

Member of these Collections

• //function/reporter/light
• iGEM Parts Registry