Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 05:57:18
sfGFP under rhamnose promoter with removed EcoRI site
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1741005_sequence (Version 1) |
Description
rhaBAD promoter has been copied from E. coli genome: ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcGAATTCaggcgctttttagactggtcgtaatgaaattcagcaggatcacattthen Eco RI site was removed by PCR
to obtain Rha1 in a biobrick standard:
ggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcGAATTTaggcgctttttagactggtcgtaatgaaattcagcaggatcacatt
The only one point mutation makes the promoter slightly stronger and more sensitive to rhamnose.
Notes
We have removed EcoRI site.Source
All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| CRP binding site CRP binding site RhaS binding site RhaS binding site -35 signal -10 signal modified 5 His-tag sfGFP two consecutive STOP codons | 1,19 30,51 52,68 85,101 99,104 122,127 138,156 157,186 187,900 901,906 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/tag tag feature/protein CDS stop_codon feature/stop |