Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-26 08:00:23
sfGFP under short rhamnose promoter (very weak)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1741006_sequence (Version 1) |
Description
From rhaBAD1 promoter we removed both CRP binding sites. It seems likely thatt RhaS binding site has been also affected, so however the resulting promoter is still induced by rhamnose, the induction is very weak.Notes
We removed both CPR sites.Source
All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| RhaS binding site RhaS binding site -35 signal -10 signal modified 5'UTR His-tag sfGFP two consecutive STOP codons | 1,17 34,50 48,53 71,76 87,105 106,135 136,849 850,855 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/tag tag feature/protein CDS feature/stop stop_codon |