BBa_K1742009

BBa_K1742009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1742009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pilar Baldominos

Date created: 2015-09-06 11:00:00

Date modified: 2015-12-16 01:53:49

35S:BxbI:T35S - 35S:ReporterBxBI::GFP:T35S

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1742009_sequence (Version 1)

Description

Composite composed of the recombinase Bxb1 optimize for Nicothiana Benthamiana and a reporter to prove its action. The reporter composite consist in a terminator flankeated by the recognition sequence of Bxb1 between the promoter and the CDS. Only if the recombinase is present, the GFP will be observed.

Notes

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Source

P35s: BBa_K1537015
Bxb1: BBa_K1742005
Bxb1reporter: BBa_K1742006
GFP: Golden braid 2.0 collection
T35s: Golden braid 2.0 collection

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1742006	1,397	engineered_region, feature/BioBrick
BBa_K1742005	404,2096	engineered_region, feature/BioBrick
35s promoter	2105,2946	promoter, feature/promoter
translation initiation optimized sequence for dicot	2947,2955	engineered_region, feature/BioBrick
mass translation enhancer	2956,2964	engineered_region, feature/BioBrick
BBa_K1537015	2105,2964	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1742009/1

Member of these Collections

• //chassis/eukaryote/nbenthamiana
• iGEM Parts Registry