BBa_K1753000

BBa_K1753000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1753000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Da Di

Date created: 2015-09-14 11:00:00

Date modified: 2015-09-15 08:05:50

the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1753000_sequence (Version 1)

Description

this part is the omega subunit of E_coli RNA polymerase, upstream of which is T7 promoter and RBS for in vitro transcription and translation system (NEB PURExpress),downstream is BBa_B0014(double terminator),between the RBS and the terminator is NdeI and NheI restriction site.
the total scheme is :
T7promoter-RBS-NdeI site-omega subunit-NheI site-BBa_B0014

Notes

we direct synthesis this part

Source

E.coli

Components

Access	Instance	Definition
public	BBa_B0014	BBa_B0014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0014	1,95	BBa_B0014

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1753000/1

Member of these Collections

• iGEM Parts Registry