VR8

BBa_K176052 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176052
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiayi Dou, Hao Jiang
Date created: 2009-08-12 11:00:00
Date modified: 2015-05-08 01:11:02

Reverse primer VR8 (bind to K176042), member of VR3~VR12



Types
DnaRegion

Roles
primer

Primer

Sequences BBa_K176052_sequence (Version 1)

Description

This part binds to K176042, which can be ligated into composite parts as a primer binding site for PCR identification of the part or sequencing of the part. It will be very useful when many composite parts have similar lengths, then PCR using VF2 and VR cannot be used to identify the parts.

Reverse primers VR3~VR12 are designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. These primers are designed to be perfectly paired with VF2 and without cross-reactivities to each other.

Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.

Source

Reverse complement of K176042. Picked from random sequences.

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K176052/1