BBa_K1763003

BBa_K1763003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1763003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinson Lam
Date created: 2015-06-01 11:00:00
Date modified: 2016-02-10 11:34:10

Major Spidroin Protein 2 (MaSp2) with sticky ends BC



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K1763003_sequence (Version 1)

Description

This part contains the core sequence of MaSp2 that has been assembled with a 5'-TGTC-3' overhang on the 5' end of the sequence and 3'-GCAC-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.

Notes

The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.

Source

This part is derived from the MaSp2 gene found in the spider N. Clavipes.

Sequence Annotation Location Component / Role(s)
BsaI recognition site (forward)
BsaI reconition site (reverse)
B sticky end
C sticky end
MaSp2 Core Sequence
1,6
114,119
7,10
109,112
10,108
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/dna
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1763003/1