BBa_K1772003

BBa_K1772003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1772003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianyu Tan
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-17 11:41:36

Lignin Peroxidase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1772003_sequence (Version 1)

Description

Protein coding sequence of Lignin Peroxidase, an enzyme that is the initiator and one of the main enzymes responsible for the breakdown of lignin inside the cell walls of plant cells. Reticulitermes flavipes, or the eastern subterranean termite, uses this enzyme to digest woody debris with higher efficiency. Lignin peroxidase has the ability to oxidize aromatic compounds in lignin with redox potentials above 1.4 eV, which is the equivalent to reducing metals like gold or molecules such as lead oxide. It is believed that this action is done by catalyzing the transfer of an oxygen atom from a hydrogen peroxide molecule to an aromatic ring. However, the exact redox mechanism for lignin peroxidase is currently unknown.

Notes

This gene sequence excludes introns (noncoding segments) present in the original gene from Reticulitermes flavipes. Sequence optimization was done using IDT's codon optimizer. Illegal restriction enzyme sites (under RFC 10 definition) were detected using NEBcutter2 and removed with silent mutations.

Source

Reticulitermes flavipes (GenBank Accession JX513905.1)

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1772003/1