Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: HKU iGEM 2015
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-20 02:01:34
CRISPR-Cas9 contaiment device repressed by arabinose and tryptophan
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K1774000_sequence (Version 1) |
Description
This is a prototype containment device utilizing CRISPR-Cas9 system which aims to control accidental leakage of GMM once it escape from medium containing arabinose and tryptophan. The device consists of two modules, firstly a arabinose repressible cas9 expression module and a tryptophan repressible gRNA expression module. Under the presence of sufficient amount of arabinose and tryptophan the device remain silent, however when bacteria escape to environment without arabinose and tryptophan, Cas9 and a gRNA targeting DNA polymerase III alpha subunit will be expressed. Subsequent cleavage of the targeted sequence may hinder DNA replication andNotes
a lotSource
Cas9 originated from S. pyogenes SF 370| Access | Instance | Definition |
| public public public public public public public public | BBa_K1333315 BBa_Q04510 BBa_B0034 BBa_K1774001 BBa_B0015 BBa_J23106 BBa_K1774003 BBa_K1774002 | BBa_K1333315 BBa_Q04510 BBa_B0034 BBa_K1774001 BBa_B0015 BBa_J23106 BBa_K1774003 BBa_K1774002 |