BBa_K1783000

BBa_K1783000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1783000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Iowis Zhu

Date created: 2015-06-02 11:00:00

Date modified: 2015-07-01 08:39:40

Miraculin Generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K1783000_sequence (Version 1)

Description

pBAD-B0034-ExpTag-Linker-Miraculin-Linker-6xHis. Adds a promoter to BBa_K1033120 in order to characterize it. Under arabinose induction, produces miraculin connected to an export tag and a His-tag via unstructured linkers. Miraculin is a taste modifying protein.

Notes

Parts were assembled using 3A assembly.

Source

All parts are from previous BioBricks. pBAD promoter is from E. coli, while Miraculin gene comes from S. dulcificum.

Components

Access	Instance	Definition
public	BBa_K206000	pBAD
public	BBa_K1033120	BBa_K1033120

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K206000	1,130	pBAD
BBa_K1033120	139,855	BBa_K1033120

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1783000/1

Member of these Collections

• iGEM Parts Registry