Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM15_Tsinghua
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 07:00:13
Blue Light Sensing System with GFP and RFP Reporter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1797003_sequence (Version 1) |
Description
This part is one of the many bio-bricks used to test the blue-light system in our project. Specifically, it contains an entire blue-light system, including a light sensor YF1 and a response regulator FixJ, and two Fixk2 promoter regulated reporters. We used this part to test the efficacy and efficiency of the blue-light system in E. coli. If the bacteria carrying this plasmid is provided with blue light, two reporters cannot be synthesized. On the contrary, if it is kept in dark, then the bacteria colony will display a mixture color from RFP and GFP.Notes
No.Source
Blue-light-sensitive LOV domain in the light sensor YF1 is cloned from a protein called YtvA from B. subtilis. HK domain in the light sensor YF1 and the response regulator FixJ are both cloned from a protein called FixL from B. japonicum.| Access | Instance | Definition |
| public public public public | BBa_J23104 BBa_B0032 BBa_E1010 BBa_K952003 | BBa_J23104 BBa_B0032 mRFP1 BBa_K952003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23104 BBa_B0032 BBa_E1010 BBa_K952003 | 1,35 44,56 63,768 777,3535 | BBa_J23104 BBa_B0032 mRFP1 BBa_K952003 |