Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Richard Wilson, Stephanie Chambers
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:06
Rock-paper-scissors - Scissors primary plasmid
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K180017_sequence (Version 1) |
Description
Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand.Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.
Notes
BBa_K180018 reading along reverse strand, and BBa_K180019 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.Source
See relevant biobrick component parts (BBa_K180018 and BBa_K180019).| Sequence Annotation | Location | Component / Role(s) |
| B0015 K180018 lasR C0179 B0034 conserved J23119 -10 -35 lacIq K180000 K180019 Ptac B0034 conserved conserved K180009 mBanana B0034 C0161 lacI B0015 | 1,129 1,921 138,860 138,860 867,878 871,874 887,921 893,898 916,921 930,2011 930,2376 930,3858 2254,2281 2385,2396 2389,2392 2390,2393 2403,3110 2403,3110 3119,3130 3137,3721 3137,3721 3730,3858 | engineered_region feature/BioBrick engineered_region feature/BioBrick feature/cds CDS feature/BioBrick engineered_region feature/BioBrick engineered_region feature/conserved conserved_region engineered_region feature/BioBrick feature/promoter promoter promoter feature/promoter CDS feature/protein feature/BioBrick engineered_region feature/BioBrick engineered_region promoter feature/promoter engineered_region feature/BioBrick conserved_region feature/conserved conserved_region feature/conserved engineered_region feature/BioBrick feature/protein CDS feature/BioBrick engineered_region feature/BioBrick engineered_region feature/cds CDS feature/BioBrick engineered_region |