BBa_K1819001

BBa_K1819001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1819001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lais Ribovski

Date created: 2015-09-12 11:00:00

Date modified: 2015-09-13 08:46:53

Latex Clearing Protein (Lcp) - rubber degradation

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1819001_sequence (Version 1)

Description

Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.

Notes

Because Lcp is secreted by Streptomyces sp, it contains a 5???-signal peptide that was identified using signal peptide predictor server (4) (http://www.compgen.org/tools/PRED-TAT). We removed this region from Lcp to generate this part flanked by SacI and NdeI restrictions sites.

Source

Lcp from actinomycete Streptomyces sp. strain K30

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Lcp	7,1132	CDS, feature/protein
SacI site	1,6	sequence_feature, feature/misc
NdeI site	1133,1138	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1819001/1

Member of these Collections

• //cds/enzyme
• iGEM Parts Registry