Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenbo Xu, Hao Wu
Date created: 2015-09-05 11:00:00
Date modified: 2015-09-17 07:15:49
Consensus Promoter T7 + RNA Thermometer A1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1824006_sequence (Version 1) |
Description
This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer A1 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequenceSource
T7 promoter was from registry.| Access | Instance | Definition |
| public public | BBa_J64997 BBa_K1824558 | BBa_J64997 BBa_K1824558 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J64997 BBa_K1824558 | 1,19 20,71 | BBa_J64997 BBa_K1824558 |