Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Saishreyas Gourishankar Iyer
Date created: 2016-10-04 11:00:00
Date modified: 2016-10-05 06:55:11
RNA thermometer + cjBlue
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1826005_sequence (Version 1) |
Description
This part is a composite of RNA thermometer and cjBlue, green chromoprotein. The RNA thermometer is an untranslated RNA sequence that will allow translation only above 42??C. Below this temperature, the RNA thermometer sequence will fold into a complex structure and prevent translation. The cjBlue , green chromoprotein is from the Cnidopus japonicus sea anemone. It naturally exhibits dark green color when expressed. The cjBlue protein is readily expressed in E.coli. The cjBlue acts as a reporter gene and the RNA thermometer, as a temperature controlled switch.Notes
Since the RNA thermometer sequence was too small, we had to keep it in its backbone and ligate cjBlue to it.Source
The cjBlue , green chromoprotein is from the Cnidopus japonicus sea anemone. RNA thermometer is from Bradyrhizobium japonicum that initiates translation at 42??C.| Access | Instance | Definition |
| public public | BBa_K115001 BBa_K592011 | BBa_K115001 cjBlue |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K115001 BBa_K592011 | 1,96 103,804 | BBa_K115001 cjBlue |