BBa_K1838001

BBa_K1838001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1838001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Connor McFadden
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-25 09:48:36

Glucose Sensitive Promoter via MLC E. coli interactions



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1838001_sequence (Version 1)

Description

This part contains the palindromic binding site for the protein MLC encoded by the gene dgsA, a gene native to E. coli. Specifically, MLC is a repressor regulator for many phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems (PTSs). In this part, the binding site is found downstream of the base promoter used for construction, BBa_J23119.

Notes

One issue to consider was where to place the MLC binding site and what strength promoter to use as a base. This was solved by creating multiple variants of the promorer (MLC1-4).

Source

1. Plumbridge, Jacqueline. ???DNA Binding Sites for the MLC and NagC Proteins: Regulation of nagE, Encoding the N-Acetylglucosamine-Specific Transporter inEscherichia Coli.??? Nucleic Acids Research 29.2 (2001): 506???514. Print.

2. http://parts.igem.org/Part:BBa_J23119

Sequence Annotation Location Component / Role(s)
BBa_J23119
MLC Binding Site
1,33
34,55
feature/promoter promoter
operator feature/operator
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1838001/1