Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iain Galpin
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 03:12:20
Lpp-OmpA sZF14
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1855003_sequence (Version 1) |
Description
A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF14),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TCCTCACTC on the cell surface of E.coli.Notes
We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF14 domain is preceeded by an NheI cut site and followed by a ClaI cut site.Source
Lpp-OmpA is originally from E.coli; sZF2 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html| Sequence Annotation | Location | Component / Role(s) |
| pL_lac B0034 Strong RBS Lpp-OmpA Linker sZF14 Linker 3x FLAG BBa_K864600 T0 Terminator | 1,56 60,80 84,518 522,557 564,833 840,863 867,938 945,996 | promoter feature/promoter ribosome_entry_site feature/rbs feature/protein CDS sequence_feature feature/misc feature/protein CDS feature/misc sequence_feature tag feature/tag stop_codon feature/stop |