| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K187002_sequence (Version 1)
|
Description
HemF encodes coproporphyrinogen III oxidase, an enzymes involved in the heme biosynthesis pathway. HemF is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see its BBF RFC.
Notes
Note that pAB does not include a promoter or terminator. pAB does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express HemF, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto HemF. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.
Source
E.coli genomic DNA
